Learn about our RNA Sequencing services.
Learn about our Whole Genome Sequencing services.
Learn about our Amplicon Sequencing services.
Learn about our Targeted Sequencing services.
Learn about our Metagenomics services.
Learn about our Epigenomics services.
Learn more our Immunogenomics services.
Learn more about our Sequencing Only service.
Learn more about our Exome Sequencing service.
Learn more about our CRISPR Validation service.
Learn more about our Digital Spatial Profiling service.
Sample Type: Cell Pellet
Recommended Amount:
≥10^6 cells (eukaryotic); ≥10^8 cells (microorganism)
Minimum Amount:
10^4 cells (eukaryotic);10^6 cells (microorganism)
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank
Sample Type: Cell Pellet
Recommended Amount: >1M cells
Minimum Amount: 30,000 cells
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Sample Type: Cells in Solution
Recommended Amount: >1,000 cells
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq
Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.
Sample Type: ChIP DNA
Recommended Amount: >50 ng
Minimum Amount: 10 ng
Fragment Length: 100-800 bp, main peak at 200-300 bp
Purity (A260/280): 1.8-2.0
Buffer: Water, BE, or low TE (<0.1 mM EDTA)
Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Sample Type: FFPE
Recommended Amount: ≥4 FFPE slides
Minimum Amount: 2 FFPE slides
Thickness: 5-20 µm
Area: >150 mm^2
Shipment Method: Room temperature or blue ice
Need additional samples for your cohort study?
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Sample Type: Fixed Needle Core Biopsy
Recommended Amount: ≥4 biopsies
Minimum Amount: 1 biopsy
Diameter: 1-2 mm
Length: >5 mm
Shipment Method: Room temperature or blue ice
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Sample Type: Fresh Frozen Tissue
Recommended Amount: >10 mg
Minimum Amount: 2 mg
Shipment Method: Dry ice
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Need additional samples for your cohort study?
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Sample Type: Frozen cell suspension according to GENEWIZ-supplied protocol, submitted in duplicate. Methanol-fixed cells are also accepted. Tissue dissociation may be available for certain samples (please inquire if interested).
Recommended Amount: >1M cells in 1 mL
Minimum Amount: 50,000 cells in 500 µL
Buffer: Cryopreservation media (e.g. cell culture media supplemented with 20% FBS and 10% DMSO)
Note: GENEWIZ has developed a proprietary cryopreservation protocol to aid shipment of samples.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice overnight
Tech Note: Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal
Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how GENEWIZ scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.
Sample Type: Frozen cell suspension according to GENEWIZ-supplied protocol
Recommended Amount: 3 tubes of >200,000 cells in 500 μL each
Minimum Amount: 3 tubes of 100,000 cells in 500 µL each (less can be accepted if necessary; please inquire)
Buffer: Cryopreservation media (e.g. cell culture media supplemented with 10% DMSO)
Note: GENEWIZ has developed a proprietary cryopreservation protocol to aid shipment of samples.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice overnight
Sample Type: Frozen intact plant tissue is preferable. Tissue ground to a fine powder can be submitted after consultation with GENEWIZ.
Amount: Very little frozen tissue is required; the equivalent of a microcentrifuge tube filled with tissue should be sufficient
Note: Samples cannot be submitted in solution, such as RNAlater.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice overnight
Sample Type: Frozen intact plant tissue is preferable. Tissue ground to a fine powder can be submitted after consultation with GENEWIZ.
Amount: 1 gram of frozen tissue is required for up to 4 immunoprecipitations (i.e. antibodies)
Note: Samples cannot be submitted in solution, such as RNAlater.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice overnight
Sample Type: Genomic DNA
Recommended Amount: >1.5 µg @ >20 ng/µL
Minimum Amount: 500 ng
Purity (A260/280): 1.8-2.0
Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank
Sample Type: Genomic DNA
Recommended Amount: >25 µg @ 100 ng/µL
Minimum Amount: 15 µg (singleplex), 5 µg (multiplex)
Purity (A260/280): 1.8-2.0
Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
View our Best Practices Guide to Preparation of High Molecular Weight DNA
Sample Type: Genomic DNA
Recommended Amount: >1 µg @ >20 ng/µL
Minimum Amount: 100 ng
Purity (A260/280): 1.8-2.0
Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank
Sample Type: Plasma/Serum
Recommended Amount: 2 tubes/sample, 10 mL/tube
Minimum Amount: 5 mL/tube
Shipment Method: Dry ice
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Need additional samples for your cohort study?
Get streamlined access to Nashville Bio's world-class biobank
Sample Type: Premade Sequencing Library
Recommended Amount: >15 nM, >20 µL
Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Shipment Method: Dry ice
Sample Type: Purified Mixed DNA
Minimum Amount: 500 ng
Concentration: Normalized to 20 ng/µL
Purity (A260/280): 1.8-2.0
Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Room temperature or blue ice
Sample Type: Purified Mixed Genomic DNA
Recommended Amount: >1.5 µg @ >20 ng/µL
Minimum Amount: 500 ng
Purity (A260/280): 1.8-2.0
Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Sample Type: Purified PCR Products
Recommended Amount: >1.5 µg @ >20 ng/µL
Minimum Amount: 500 ng
Purity (A260/280): 1.8-2.0
Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Sample Type: Purified PCR Products for PacBio
Recommended Amount: >2 µg @ >50 ng/µL
Purity (A260/280): 1.8-2.0
Buffer: 10 mM Tris-HCl, pH 8
Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
View our Best Practices Guide to Preparation of Amplicon DNA for PacBio Sequencing
Sample Type: Purified PCR Products
Size: 150-500 bp
For optimal results, we recommend samples that produce only one band on a gel.
Minimum Amount: 500 ng
We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.
Concentration: Normalized to 20 ng/µL
Purity (A260/280): 1.8-2.0
Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Submission Methods:
View our Amplicon-EZ Best Practices Checklist for more info
Sample Type: Purified Small RNA
Recommended Amount: >50 ng @ >5 ng/µL
Purity (A260/280): 1.8-2.2
Recommended RIN: ≥6.0
Buffer: Nuclease-free water
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Sample Type: Saliva
Recommended Amount: 1 mL
Shipment Method: Dry ice or blue ice
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Sample Type: Snap Frozen Needle Core Biopsy
Recommended Amount: 10 mg
Minimum Amount: 3 mg
Shipment Method: Dry ice
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Sample Type: Soil
Recommended Amount: 100 mg
Minimum Amount: 50 mg
Shipment Method: Room temperature or blue ice
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Sample Type: Stool
Recommended Amount: 100 mg
Minimum Amount: 50 mg
Shipment Method: Dry ice or blue ice
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Sample Type: Swab
Recommended Amount: 100 mg
Shipment Method: Room temperature
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Sample Type: Total RNA
Recommended Amount: >2 µg @ >50 ng/µL
Minimum Amount: 500 ng
Purity (A260/280): 1.8-2.2
Recommended RIN: ≥6.0
Buffer: Nuclease-free water
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice or RNA Stabilization Tubes (see below)
Sample Type: Total RNA (Iso-Seq)
Recommended Amount: >2 µg, 10-20 µL
Purity (A260/280): 1.8-2.2
Recommended RIN: ≥9.0
Buffer: Nuclease-free water
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Sample Type: Total RNA (ultra-low input)
Recommended Amount: 20 ng
Buffer: Nuclease-free water
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq
Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.
Sample Type: Total RNA, including small RNA
Recommended Amount: >2 µg @ >50 ng/µL
Minimum Amount: 500 ng
Purity (A260/280): 1.8-2.2
Recommended RIN: ≥6.0
Buffer: Nuclease-free water
Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.
Shipment Method: Dry ice
Sample Type: Water (environmental)
Recommended Volume: 100 mL
Minimum Volume: 20 mL
Shipment Method: Room temperature
Sample Type: Whole Blood (DNA extraction)
Recommended Amount: 2 tubes/sample, 500 µL/tube
Minimum Amount: 200 µL/tube
Shipment Method:
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Need additional samples for your cohort study?
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Sample Type: Whole Blood (RNA extraction)
Recommended Volume: 2.5 mL in PAXgene Blood RNA Tube
Shipment Method:
Note: GENEWIZ can work with BSL1 and some BSL2 specimens.
Need additional samples for your cohort study?
Get streamlined access to Nashville Bio's world-class biobank
Sample Type: FFPE Blocks
Recommended Amount: 1 block per sample
Minimum Volume: 1 block per sample
Shipment Method: Room temperature
Sample Type: FFPE Sections
Thickness: 5 µm (on positively charged slides)
Recommended Amount: 2 slides per sample, each with a single serial section, together with a serial H&E section annotated with the regions of interest to be profiled
Minimum Volume: 1 slide per sample
Shipment Method: Room temperature, in a slide mailer tube (to protect slides from damage)
Sample Type: Frozen Sections
Thickness: 5 µm (on positively charged slides)
Recommended Amount: 2 slides per sample, each with a single serial section, together with a serial H&E section annotated with the regions of interest to be profiled
Minimum Volume: 1 slide per sample
Shipment Method: Dry ice overnight, in a slide mailer tube (to protect slides from damage)