Step 1: Select the appropriate service and sample type.

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Learn about our RNA Sequencing services.

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Learn about our Whole Genome Sequencing services.

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Learn about our Amplicon Sequencing services.

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Learn about our Targeted Sequencing services.

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Learn about our Metagenomics services.

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Learn about our Epigenomics services.

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Learn more our Immunogenomics services.

Learn more about our Sequencing Only service.

Learn more about our Exome Sequencing service.

Learn more about our CRISPR Validation service.

Learn more about our Digital Spatial Profiling service.

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Step 2: Follow these guidelines when preparing and submitting your samples.

Cell Pellet

Sample Type: Cell Pellet

Recommended Amount: 

≥10^6 cells (eukaryotic); ≥10^8 cells (microorganism)

Minimum Amount:

10^4 cells (eukaryotic);10^6 cells (microorganism)

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

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Cell Pellet (genoTYPER-NEXT)

Sample Type: Cell Pellet

Recommended Amount: >1M cells

Minimum Amount: 30,000 cells

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Cells in Solution

Sample Type: Cells in Solution

Recommended Amount: >1,000 cells

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

 

 

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Download >

 

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ChIP DNA

Sample Type: ChIP DNA

Recommended Amount: >50 ng

Minimum Amount: 10 ng

Fragment Length: 100-800 bp, main peak at 200-300 bp

Purity (A260/280): 1.8-2.0

Buffer: Water, BE, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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FFPE

Sample Type: FFPE

Recommended Amount: ≥4 FFPE slides

Minimum Amount: 2 FFPE slides

Thickness: 5-20 µm

Area: >150 mm^2

Shipment Method: Room temperature or blue ice

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

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Fixed Needle Core Biopsy

Sample Type: Fixed Needle Core Biopsy

Recommended Amount: ≥4 biopsies

Minimum Amount: 1 biopsy

Diameter: 1-2 mm

Length: >5 mm

Shipment Method: Room temperature or blue ice

 

 

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Fresh Frozen Tissue

Sample Type: Fresh Frozen Tissue

Recommended Amount: >10 mg

Minimum Amount: 2 mg

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

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Single-Cell

Sample Type: Frozen cell suspension according to GENEWIZ-supplied protocol, submitted in duplicate. Methanol-fixed cells are also accepted. Tissue dissociation may be available for certain samples (please inquire if interested).

Recommended Amount: >1M cells in 1 mL

Minimum Amount: 50,000 cells in 500 µL

Buffer: Cryopreservation media (e.g. cell culture media supplemented with 20% FBS and 10% DMSO)

 

Note: GENEWIZ has developed a proprietary cryopreservation protocol to aid shipment of samples.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

 

 

Tech Note: Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal

Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how GENEWIZ scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.

Download >

 

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ATAC-Seq Cells

Sample Type: Frozen cell suspension according to GENEWIZ-supplied protocol

Recommended Amount: 3 tubes of >200,000 cells in 500 μL each

Minimum Amount: 3 tubes of 100,000 cells in 500 µL each (less can be accepted if necessary; please inquire)

Buffer: Cryopreservation media (e.g. cell culture media supplemented with 10% DMSO)

 

Note: GENEWIZ has developed a proprietary cryopreservation protocol to aid shipment of samples.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

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ATAC-Seq Plant

Sample Type: Frozen intact plant tissue is preferable. Tissue ground to a fine powder can be submitted after consultation with GENEWIZ.

Amount: Very little frozen tissue is required; the equivalent of a microcentrifuge tube filled with tissue should be sufficient

Note: Samples cannot be submitted in solution, such as RNAlater.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

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ATAC-Seq Plant

Sample Type: Frozen intact plant tissue is preferable. Tissue ground to a fine powder can be submitted after consultation with GENEWIZ.

Amount: 1 gram of frozen tissue is required for up to 4 immunoprecipitations (i.e. antibodies)

 

Note: Samples cannot be submitted in solution, such as RNAlater.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

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Genomic DNA

Sample Type: Genomic DNA

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

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Genomic DNA PacBio

Sample Type: Genomic DNA

Recommended Amount: >25 µg @ 100 ng/µL

Minimum Amount: 15 µg (singleplex), 5 µg (multiplex)

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

View our Best Practices Guide to Preparation of High Molecular Weight DNA​ 

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Genomic DNA (targeted amplicon)

Sample Type: Genomic DNA

Recommended Amount: >1 µg @ >20 ng/µL

Minimum Amount: 100 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

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Plasma/Serum

Sample Type: Plasma/Serum

Recommended Amount: 2 tubes/sample, 10 mL/tube

Minimum Amount: 5 mL/tube

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

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Premade Sequencing Library

Sample Type: Premade Sequencing Library

Recommended Amount: >15 nM, >20 µL

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Shipment Method: Dry ice

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Purified Mixed DNA

Sample Type: Purified Mixed DNA

Minimum Amount: 500 ng

Concentration: Normalized to 20 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Room temperature or blue ice

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Purified Mixed Genomic DNA

Sample Type: Purified Mixed Genomic DNA

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Purified PCR Products

Sample Type: Purified PCR Products

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Purified PCR Products PacBio

Sample Type: Purified PCR Products for PacBio

Recommended Amount: >2 µg @ >50 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: 10 mM Tris-HCl, pH 8

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

View our Best Practices Guide to Preparation of Amplicon DNA for PacBio Sequencing

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Amplicon-EZ

Sample Type: Purified PCR Products

Size: 150-500 bp

For optimal results, we recommend samples that produce only one band on a gel.

Minimum Amount: 500 ng

We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Concentration: Normalized to 20 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Submission Methods: 

  • Drop off samples in a GENEWIZ drop box at room temperature
  • Ship at room temperature or on blue ice

View our Amplicon-EZ Best Practices Checklist for more info

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Purified Small RNA

Sample Type: Purified Small RNA

Recommended Amount: >50 ng @ >5 ng/µL

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Saliva

Sample Type: Saliva

Recommended Amount: 1 mL

Shipment Method: Dry ice or blue ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Snap Frozen Needle Core Biopsy

Sample Type: Snap Frozen Needle Core Biopsy

Recommended Amount: 10 mg

Minimum Amount: 3 mg

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Soil

Sample Type: Soil

Recommended Amount: 100 mg

Minimum Amount: 50 mg

Shipment Method: Room temperature or blue ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Stool

Sample Type: Stool

Recommended Amount: 100 mg

Minimum Amount: 50 mg

Shipment Method: Dry ice or blue ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Swab

Sample Type: Swab

Recommended Amount: 100 mg

Shipment Method: Room temperature

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Total RNA

Sample Type: Total RNA

Recommended Amount: >2 µg @ >50 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

 

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice or RNA Stabilization Tubes (see below)

Also, you can order RNA Stabilization Tubes through our online store. Learn more.

 

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Total RNA (Iso-Seq)

Sample Type: Total RNA (Iso-Seq)

Recommended Amount: >2 µg, 10-20 µL

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥9.0

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Total RNA (ultra-low input)

Sample Type: Total RNA (ultra-low input)

Recommended Amount: 20 ng

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

 

 

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Download >

 

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Total RNA (small RNA)

Sample Type: Total RNA, including small RNA

Recommended Amount: >2 µg @ >50 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

 

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Water

Sample Type: Water (environmental)

Recommended Volume: 100 mL

Minimum Volume: 20 mL

Shipment Method: Room temperature

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Whole Blood (DNA)

Sample Type: Whole Blood (DNA extraction)

Recommended Amount: 2 tubes/sample, 500 µL/tube

Minimum Amount: 200 µL/tube

Shipment Method:

  • Blue ice for fresh blood
  • Dry ice for frozen blood

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

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Whole Blood (RNA)

Sample Type: Whole Blood (RNA extraction)

Recommended Volume: 2.5 mL in PAXgene Blood RNA Tube

Shipment Method:

  • Blue ice for fresh blood
  • Dry ice for frozen blood

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

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Water

Sample Type: FFPE Blocks

Recommended Amount: 1 block per sample

Minimum Volume: 1 block per sample

Shipment Method: Room temperature

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Water

Sample Type: FFPE Sections

Thickness: 5 µm (on positively charged slides)

Recommended Amount: 2 slides per sample, each with a single serial section, together with a serial H&E section annotated with the regions of interest to be profiled

Minimum Volume: 1 slide per sample

Shipment Method: Room temperature, in a slide mailer tube (to protect slides from damage)

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Water

Sample Type: Frozen Sections

Thickness: 5 µm (on positively charged slides)

Recommended Amount: 2 slides per sample, each with a single serial section, together with a serial H&E section annotated with the regions of interest to be profiled

Minimum Volume: 1 slide per sample

Shipment Method: Dry ice overnight, in a slide mailer tube (to protect slides from damage)

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< Start Over

Step 1: Select the appropriate service and sample type.

NGS Category {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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RNA Sequencing Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Learn about our RNA Sequencing services.

Whole Genome Sequencing Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Learn about our Whole Genome Sequencing services.

Amplicon Sequencing Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Learn about our Amplicon Sequencing services.

Targeted Sequencing Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Learn about our Targeted Sequencing services.

Metagenomics Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Learn about our Metagenomics services.

Epigenomics Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Learn about our Epigenomics services.

Immunogenomics Services {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Learn more our Immunogenomics services.

Learn more about our Sequencing Only service.

Learn more about our Exome Sequencing service.

Learn more about our CRISPR Validation service.

Learn more about our Digital Spatial Profiling service.

Sample Type (RNA-Seq) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Sample Type (Small RNA-Seq) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Sample Type (Ultra-Low Input RNA-Seq) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Sample Type (Whole Genome) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Sample Type (Targeted/Exome) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Sample Type (Whole Genome Metagenomics) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Sample Type (genoTYPER-NEXT) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Sample Type (16S-EZ/Fungal ID) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Sample Type (Bulk Immuno-Profiling) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Sample Type (ATAC-Seq) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Sample Type (Digitial Spatial Profiling) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Sample Type (ChIP-Seq) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
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Step 2: Follow these guidelines when preparing and submitting your samples.

Cell Pellet

Sample Type: Cell Pellet

Recommended Amount: 

≥10^6 cells (eukaryotic); ≥10^8 cells (microorganism)

Minimum Amount:

10^4 cells (eukaryotic);10^6 cells (microorganism)

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

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Cell Pellet (genoTYPER-NEXT)

Sample Type: Cell Pellet

Recommended Amount: >1M cells

Minimum Amount: 30,000 cells

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Cells in Solution

Sample Type: Cells in Solution

Recommended Amount: >1,000 cells

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

 

 

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Download >

 

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ChIP DNA

Sample Type: ChIP DNA

Recommended Amount: >50 ng

Minimum Amount: 10 ng

Fragment Length: 100-800 bp, main peak at 200-300 bp

Purity (A260/280): 1.8-2.0

Buffer: Water, BE, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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FFPE

Sample Type: FFPE

Recommended Amount: ≥4 FFPE slides

Minimum Amount: 2 FFPE slides

Thickness: 5-20 µm

Area: >150 mm^2

Shipment Method: Room temperature or blue ice

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

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Fixed Needle Core Biopsy

Sample Type: Fixed Needle Core Biopsy

Recommended Amount: ≥4 biopsies

Minimum Amount: 1 biopsy

Diameter: 1-2 mm

Length: >5 mm

Shipment Method: Room temperature or blue ice

 

 

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Fresh Frozen Tissue

Sample Type: Fresh Frozen Tissue

Recommended Amount: >10 mg

Minimum Amount: 2 mg

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

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Single-Cell

Sample Type: Frozen cell suspension according to GENEWIZ-supplied protocol, submitted in duplicate. Methanol-fixed cells are also accepted. Tissue dissociation may be available for certain samples (please inquire if interested).

Recommended Amount: >1M cells in 1 mL

Minimum Amount: 50,000 cells in 500 µL

Buffer: Cryopreservation media (e.g. cell culture media supplemented with 20% FBS and 10% DMSO)

 

Note: GENEWIZ has developed a proprietary cryopreservation protocol to aid shipment of samples.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

 

 

Tech Note: Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal

Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how GENEWIZ scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.

Download >

 

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ATAC-Seq Cells

Sample Type: Frozen cell suspension according to GENEWIZ-supplied protocol

Recommended Amount: 3 tubes of >200,000 cells in 500 μL each

Minimum Amount: 3 tubes of 100,000 cells in 500 µL each (less can be accepted if necessary; please inquire)

Buffer: Cryopreservation media (e.g. cell culture media supplemented with 10% DMSO)

 

Note: GENEWIZ has developed a proprietary cryopreservation protocol to aid shipment of samples.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

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ATAC-Seq Plant

Sample Type: Frozen intact plant tissue is preferable. Tissue ground to a fine powder can be submitted after consultation with GENEWIZ.

Amount: Very little frozen tissue is required; the equivalent of a microcentrifuge tube filled with tissue should be sufficient

Note: Samples cannot be submitted in solution, such as RNAlater.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

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ATAC-Seq Plant

Sample Type: Frozen intact plant tissue is preferable. Tissue ground to a fine powder can be submitted after consultation with GENEWIZ.

Amount: 1 gram of frozen tissue is required for up to 4 immunoprecipitations (i.e. antibodies)

 

Note: Samples cannot be submitted in solution, such as RNAlater.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

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Genomic DNA

Sample Type: Genomic DNA

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

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Genomic DNA PacBio

Sample Type: Genomic DNA

Recommended Amount: >25 µg @ 100 ng/µL

Minimum Amount: 15 µg (singleplex), 5 µg (multiplex)

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

View our Best Practices Guide to Preparation of High Molecular Weight DNA​ 

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Genomic DNA (targeted amplicon)

Sample Type: Genomic DNA

Recommended Amount: >1 µg @ >20 ng/µL

Minimum Amount: 100 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

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Plasma/Serum

Sample Type: Plasma/Serum

Recommended Amount: 2 tubes/sample, 10 mL/tube

Minimum Amount: 5 mL/tube

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

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Premade Sequencing Library

Sample Type: Premade Sequencing Library

Recommended Amount: >15 nM, >20 µL

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Shipment Method: Dry ice

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Purified Mixed DNA

Sample Type: Purified Mixed DNA

Minimum Amount: 500 ng

Concentration: Normalized to 20 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Room temperature or blue ice

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Purified Mixed Genomic DNA

Sample Type: Purified Mixed Genomic DNA

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Purified PCR Products

Sample Type: Purified PCR Products

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Purified PCR Products PacBio

Sample Type: Purified PCR Products for PacBio

Recommended Amount: >2 µg @ >50 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: 10 mM Tris-HCl, pH 8

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

View our Best Practices Guide to Preparation of Amplicon DNA for PacBio Sequencing

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Amplicon-EZ

Sample Type: Purified PCR Products

Size: 150-500 bp

For optimal results, we recommend samples that produce only one band on a gel.

Minimum Amount: 500 ng

We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Concentration: Normalized to 20 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Submission Methods: 

  • Drop off samples in a GENEWIZ drop box at room temperature
  • Ship at room temperature or on blue ice

View our Amplicon-EZ Best Practices Checklist for more info

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Purified Small RNA

Sample Type: Purified Small RNA

Recommended Amount: >50 ng @ >5 ng/µL

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Saliva

Sample Type: Saliva

Recommended Amount: 1 mL

Shipment Method: Dry ice or blue ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Snap Frozen Needle Core Biopsy

Sample Type: Snap Frozen Needle Core Biopsy

Recommended Amount: 10 mg

Minimum Amount: 3 mg

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Soil

Sample Type: Soil

Recommended Amount: 100 mg

Minimum Amount: 50 mg

Shipment Method: Room temperature or blue ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Stool

Sample Type: Stool

Recommended Amount: 100 mg

Minimum Amount: 50 mg

Shipment Method: Dry ice or blue ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Swab

Sample Type: Swab

Recommended Amount: 100 mg

Shipment Method: Room temperature

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

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Total RNA

Sample Type: Total RNA

Recommended Amount: >2 µg @ >50 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

 

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice or RNA Stabilization Tubes (see below)

Also, you can order RNA Stabilization Tubes through our online store. Learn more.

 

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Total RNA (Iso-Seq)

Sample Type: Total RNA (Iso-Seq)

Recommended Amount: >2 µg, 10-20 µL

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥9.0

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Total RNA (ultra-low input)

Sample Type: Total RNA (ultra-low input)

Recommended Amount: 20 ng

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

 

 

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Download >

 

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Total RNA (small RNA)

Sample Type: Total RNA, including small RNA

Recommended Amount: >2 µg @ >50 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

 

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

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Water

Sample Type: Water (environmental)

Recommended Volume: 100 mL

Minimum Volume: 20 mL

Shipment Method: Room temperature

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Whole Blood (DNA)

Sample Type: Whole Blood (DNA extraction)

Recommended Amount: 2 tubes/sample, 500 µL/tube

Minimum Amount: 200 µL/tube

Shipment Method:

  • Blue ice for fresh blood
  • Dry ice for frozen blood

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

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Whole Blood (RNA)

Sample Type: Whole Blood (RNA extraction)

Recommended Volume: 2.5 mL in PAXgene Blood RNA Tube

Shipment Method:

  • Blue ice for fresh blood
  • Dry ice for frozen blood

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

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Water

Sample Type: FFPE Blocks

Recommended Amount: 1 block per sample

Minimum Volume: 1 block per sample

Shipment Method: Room temperature

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Water

Sample Type: FFPE Sections

Thickness: 5 µm (on positively charged slides)

Recommended Amount: 2 slides per sample, each with a single serial section, together with a serial H&E section annotated with the regions of interest to be profiled

Minimum Volume: 1 slide per sample

Shipment Method: Room temperature, in a slide mailer tube (to protect slides from damage)

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Water

Sample Type: Frozen Sections

Thickness: 5 µm (on positively charged slides)

Recommended Amount: 2 slides per sample, each with a single serial section, together with a serial H&E section annotated with the regions of interest to be profiled

Minimum Volume: 1 slide per sample

Shipment Method: Dry ice overnight, in a slide mailer tube (to protect slides from damage)

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